New research on Na,K-ATPase!Knockdown of Na,K-ATPase β-subunits in Oncopeltus fasciatus induces molting problems and alterations in tracheal morphology

23 November 2022, by Marlena Herbertz

Photo: Marlena Herbertz

Knockdown of different beta-subunits in nymphs of the large milkweed bug via RNAi results in molting problems and altered tracheal morphology in adults

In this study, we analyzed the tissue-specific distribution and function of different Na,K-ATPase subunits of the large milkweed bug Oncopeltus fasciatus. We focused on the β-subunit, which functions are still not completely understood. Four β-subunits exists in O. fasciatus that were subject of our study. Function of the four β-subunits was analyzed by knocking down the genes via RNAi, which led to a suppressed protein expression. A knockdown of different β-subunits heavily interferes with molting success resulting in strongly hampered phenotypes. Remarkably, β-knockdown led to alteration in tracheal morphology. We discuss if these changes are related to disrupted cell-cell contacts.

For more information find the publication here:  https://doi.org/10.1111/1744-7917.13117