tag:www.biologie.uni-hamburg.de,2005:/en/forschung/molekular-und-zellbiologie/pflbiocheminfekt/1aktuellesNews2023-10-04T10:05:04ZNAGR-fakmin-35775622-production2023-10-03T22:00:00ZWelcome, Athina!<p>We are delighted to welcome Dr. Athina Parasyri in our group! Athina will strengthen our team as a postdoc in research and teaching in the next few years.</p>
<p>Be very welcome!</p>NAGR-fakmin-35771569-production2023-09-24T22:00:00ZWelcome to our MOPS rotation students!<p>We are delighted to welcome the two new rotation students - Silja Seemann and Vanja Gotovac - in our department. Be very welcome!</p>NAGR-fakmin-34243926-production2023-04-09T22:00:00ZWelcome, Eyleen!<p>We are delighted to welcome the bachelor student Eyleen Enke in our department. Eyleen will work on the Spirogyra project. Be very welcome!</p>NAGR-fakmin-34243880-production2023-04-02T22:00:00ZWelcome, Marcela!<p>We are delighted to welcome the new rotation student of the course Master of Molecular Plant Science Marcela De Lacerda in our department. Be very welcome!</p>NAGR-fakmin-33932938-production2023-02-28T23:00:00ZWelcome to our master students!<p>We are delighted to welcome two master students - Ipek Dinler and Lara-Marie Halscheid - in our department. Be very welcome!</p>NAGR-fakmin-33756461-production2023-02-13T23:00:00ZCongratulations, Sweta!<img width="293" height="165" style="float:left" src="https://assets.rrz.uni-hamburg.de/instance_assets/fakmin/33756550/sweta-733x414-aba34948efdcbc746a8b8914758abaa76bd98afe.jpg" /><p>On 14 February Sweta Rout successfully defended her PhD thesis"Production of recombinant proteins and omega-3 fatty acids in Nannochloropsis" . Congratulations, Sweta!</p>
<p></p>
<p></p><p>Photo: UHH/PBI</p>NAGR-fakmin-32340237-production2022-09-25T22:00:00ZParticipation in the 8th Open European Peroxisome Meeting in Aveira<p>Sigrun Reumann and the doctoral candidate Stefan Wirling participated in the 8th Open European Peroxisome Meeting on 22-24 September in Aveira, Portugal. Stefan Wirling presented his research results in a talk titled “Strategies of viruses co-opting plant peroxisomes to neutralize antiviral defense mechanisms”. They were discussed with great interest.</p>NAGR-fakmin-31994306-production2022-09-06T22:00:00ZProduction of a viral surface protein in Nannochloropsis oceanica for fish vaccination against infectious pancreatic necrosis virus<p>Nannochloropsis oceanica is a unicellular oleaginous microalga of emerging biotechnological interest with a sequenced, annotated genome, available transcriptomic and proteomic data, and well-established basic molecular tools for genetic engineering. To establish N. oceanica as a eukaryotic host for recombinant protein synthesis and develop molecular technology for vaccine production, we chose the viral surface protein 2 (VP2) of a pathogenic fish virus that causes infectious pancreatic necrosis as a model vaccine. Upon stable nuclear transformation of N. oceanica strain CCMP1779 with the codon-optimized VP2 gene, a Venus reporter fusion served to evaluate the strength of different endogenous promoters in transformant populations by qPCR and flow cytometry. The highest VP2 yields were achieved for the elongation factor promoter, with enhancer effects by its N-terminal leader sequence. Individual transformants differed in their production capability of reporter-free VP2 by orders of magnitude. When subjecting the best candidates to kinetic analyses of growth and VP2 production in photobioreactors, recombinant protein integrity was maintained until the early stationary growth phase, and a high yield of 4.4% VP2 of total soluble protein was achieved. The maximum yield correlated with multiple integrations of the expression vector into the nuclear genome. The results demonstrate that N. oceanica was successfully engineered to constitute a robust platform for high-level production of a model subunit vaccine. The molecular methodology established here can likely be adapted in a straightforward manner to the production of further vaccines in the same host, allowing their distribution to fish, vertebrates, or humans via a microalgae-containing diet.</p>
<p>Sweta Suman Rout, Imke de Grahl, Xiaohong Yu, Sigrun Reumann (2022) Production of a viral surface protein in Nannochloropsis oceanica for fish vaccination against infectious pancreatic necrosis virus Applied Genetics and Molecular Biotechnology (2022) DOI: 10.1007/s00253-022-12106-7</p>NAGR-fakmin-31807100-production2022-08-16T22:00:00ZCongratulations, Saugat!<img width="293" height="165" style="float:left" src="https://assets.rrz.uni-hamburg.de/instance_assets/fakmin/31826832/saugathut733x414-9dfbdf3d9b2e0935366dc0efe44045a2b3b3c9d3.jpg" /><p>On 17 August Saugat Pokhrel successfully defended his PhD thesis “Immune-associated small GTPases of Arabidopsis thaliana: dissecting the activation mechanism and their roles in organelle biogenesis”. Congratulations, Saugat!</p><p>Photo: UHH/PBI</p>NAGR-fakmin-30144142-production2022-04-04T22:00:00ZWelcome to our bachelor student!<p>We are delighted to welcome the bachelor student Sarah Tiedemann in our department. Sarah will work on the Arabidopsis-Fusarium project. Be very welcome!</p>NAGR-fakmin-30115140-production2022-04-04T22:00:00ZWelcome to our MOPS rotation students!<p>We are delighted to welcome the two new rotation students of the course Master of Molecular Plant Science - Lara-Marie Halscheid and Malte Rumpf - in our department. Be very welcome!</p>NAGR-fakmin-30144286-production2022-04-04T22:00:00ZWelcome, Alexander!<p>We are delighted to welcome the master student Alexander Maliskat in our department. Alexander will work on the microalgae project. Be very welcome!</p>NAGR-fakmin-29408158-production2022-01-31T23:00:00ZWelcome to our master students!<p>We are delighted to welcome the master students Chaithanya Lakshmi, Elme Tabassum and Fatemeh Mousavi in our department. Be very welcome!</p>NAGR-fakmin-29407087-production2022-01-09T23:00:00ZWelcome, Shoshant!<p>We are delighted to welcome the bachelor student Shoshant Prasad Sah in our department. Be very welcome!</p>NAGR-fakmin-29652115-production2022-01-09T23:00:00ZThe essential role of fungal peroxisomes in plant infection<p>Several filamentous fungi are ecologically and economically important plant pathogens that infect a broad variety of crops. They cause high annual yield losses and contaminate seeds and fruits with mycotoxins. Not only powerful infection structures and detrimental toxins, but also cell organelles, such as peroxisomes, play important roles in plant infection. In this review, we summarize recent research results that revealed novel peroxisomal functions of filamentous fungi and highlight the importance of peroxisomes for infection of host plants. Central for fungal virulence are two primary metabolic pathways, fatty acid β-oxidation and the glyoxylate cycle, both of which are required to produce energy, acetyl-CoA, and carbohydrates. These are ultimately needed for the synthesis of cell wall polymers and for turgor generation in infection structures. Most novel results stem from different routes of secondary metabolism and demonstrate that peroxisomes produce important precursors and house various enzymes needed for toxin production and melanization of appressoria. All these peroxisomal functions in fungal virulence might represent elegant targets for improved crop protection.</p>
<p>Falter, C., Reumann, S. (2022) The essential role of fungal peroxisomes in plant infection Mol Plant Pathol (2022) DOI: 10.1111/mpp.13180</p>NAGR-fakmin-29406900-production2021-10-14T22:00:00ZWelcome, Gabriel!<p>We are delighted to welcome the new rotation student of the international course Master of Molecular Plant Science Gabriel Méndez in our department. Be very welcome!</p>NAGR-fakmin-29592517-production2021-08-27T22:00:00ZStramenopile microalgae as "green biofactories" for recombinant protein production<p>Photoautotrophic microalgae have become intriguing hosts for recombinant protein production because they offer important advantages of both prokaryotic and eukaryotic expression systems. Advanced molecular tools have recently been established for the biotechnologically relevant group of stramenopile microalgae, particularly for several Nannochloropsis species and diatoms. Strategies for the selection of powerful genetic elements and for optimization of protein production have been reported. Much needed high-throughput techniques required for straight-forward identification and selection of the best expression constructs and transformants have become available and are discussed. The first recombinant proteins have already been produced successfully in stramenopile microalgae and include not only several subunit vaccines but also one antimicrobial peptide, a fish growth hormone, and an antibody. These research results offer interesting future applications in aquaculture and as biopharmaceuticals. In this review we highlight recent progress in genetic technology development for recombinant protein production in the most relevant Nannochloropsis species and diatoms. Diverse realistic biotechnological applications of these proteins are emphasized that have the potential to establish stramenopile algae as sustainable green factories for an economically competitive production of high-value biomolecules.</p>
<p>de Grahl, I., Reumann, S. (2021) Stramenopile microalgae as "green biofactories" for recombinant protein production World J Microbiol Biotechnol (2021) doi: 10.1007/s11274-021-03126-y</p>NAGR-fakmin-27758932-production2021-08-04T22:00:00ZCongratulations, Thu!<p>On 4 August Thu Nguyen successfully defended her PhD thesis “A peroxisomal sub-family of Arabidopsis NDR1 homologs: Molecular characterization of a novel targeting pathway and the proteins’ functions in plant immunity”. Congratulations, Thu!</p>NAGR-fakmin-29408267-production2021-04-04T22:00:00ZWelcome, Annemarie!<p>We are delighted to welcome the bachelor student Annemarie Hellberg in our department. Be very welcome!</p>