- University of Hamburg - Faculty of Biology - Biocenter Klein Flottbek
Transposition is another means of generating genetic diversity. Transposons are mobile genetic elements carrying some payload and coding the enzyme needed for their excision and reinsertion from/to the genome of the host. The procedure is universal, transposons are found from bacteria to man. According to their mechanisms transposons are sorted into families. Here the action of transposon Tn5 will be described, along with the structure of the enzyme involved.
The mechanism of Tn5 transposition may be dissected into several steps:
The transposase coded by Tn5 was crystallized in the presence of duplex DNA corresponding to the Tn5 recognition sequence. A view of a molecular model of the transposase in action is just a click away: